Effect of PGPR (Plant Growth Promoting Rhizobacteria) Concentration on the Growth of Rice Seedlings (Oryza sativa L.) IPB 3S
DOI:
https://doi.org/10.31102/agrosains.2026.11.1.105-126Keywords:
IPB 3S, PGPR, rice, vegetative growthAbstract
Plant Growth Promoting Rhizobacteria (PGPR) are beneficial microorganisms capable of enhancing plant growth by improving nutrient availability, producing phytohormones, and stimulating root development. This study aimed to evaluate the effect of different PGPR concentrations on the growth of rice seedlings (Oryza sativa L.) cultivar IPB 3S. The experiment was arranged in a Completely Randomized Design (CRD) consisting of six treatments: control (0 mL), PGPR at 25, 50, 75, 100, and 125 mL, with four replications. Growth parameters observed included plant height, leaf length, leaf width, number of leaves, root length, and fresh weight at 7, 11, 15, 19, and 23 days after sowing. Data were analyzed using analysis of variance (ANOVA), followed by the Least Significant Difference (LSD) test at the 5% significance level. The results showed that PGPR application did not significantly affect seedling growth during the initial stage (7–11 days after sowing), but significantly improved plant height, leaf length, leaf width, root length, and fresh weight from 15 days after sowing onward. The PGPR concentration of 25 mL produced the best performance for most growth parameters, whereas higher concentrations (≥75 mL did not further improve seedling growth. Therefore, the application of PGPR at 25 mL is recommended as the optimum concentration for promoting rice seedling growth during the nursery stage.
References
Afdhal, M., Safitri, T., Ayu, R. D., Salvara, D. A., & Soesanto, L. (2026). Pendekatan biological control berbasis Plant Growth Promoting Rhizobacteria (PGPR) dalam mengurangi ketergantungan pestisida kimia pada sistem produksi padi: A review article. Gontor Agrotech Science Journal, 12(1), 41–50. https://doi.org/10.21111/agrotech.v12i01.10
Asfar, A. M. I. A., Mukhsen, M. I., Rifai, A., Asfar, A. M. I. T., Asfar, A. H., Kurnia, A., & Syaifullah, A. (2022). Pemanfaatan akar bambu sebagai biang bakteri perakaran PGPR di Desa Latellang. JMM (Jurnal Masyarakat Mandiri), 6(5), 3954–3963. https://doi.org/10.31764/jmm.v6i5.10464
Ashrafuzzaman, M., Hossen, F. A., Ismail, M. R., Hoque, M. A., Islam, M. Z., Shahidullah, S. M., & Meon, S. (2009). Efficiency of plant growth-promoting rhizobacteria (PGPR) for the enhancement of rice growth. African Journal of Biotechnology, 8(7), 1247–1252. http://www.academicjournals.org/AJB
Bashan, Y., & de-Bashan, L. E. (2010). How the plant growth-promoting bacterium Azospirillum promotes plant growth—A critical assessment. Advances in Agronomy, 108, 77–136. https://doi.org/10.1016/S0065-2113(10)08002-8
Constantia, J., & Ferniah, R. S. (2020). Vegetative growth of rainbow chili (Capsicum annuum L.) in the treatment of PGPR (Plant Growth Promoting Rhizobacteria), PGPR–NPK fertilizer, and PGPR–compost combination. AGRIC, 32(2), 95–104. https://doi.org/10.24246/agric.2020.v32.i2.p95-104
Glick, B. R. (2012). Plant growth-promoting bacteria: Mechanisms and applications. Scientifica, 2012, Article 963401. https://doi.org/10.6064/2012/963401
Handoyo, G. C., Yusuf, F. H., Sugiarta, E. P., Shalom, F. H., Hidayat, N., Lestari, E. F., ... & Khorunisa, A. N. (2024). Pembuatan pupuk hayati PGPR (Plant Growth Promoting Rhizobacteria) dengan bahan dasar akar bambu di Desa Glagahwangi, Polanharjo, Klaten. Dalam Prosiding Seminar Nasional Pengabdian Masyarakat & CSR Fakultas Pertanian UNS (Vol. 4, No. 1, pp. 51–59). https://proceeding.uns.ac.id/pengabdianfp/article/view/592
Kirani, C. (2023). Peran beberapa bioaktivator sebagai pemacu viabilitas dan vigoritas benih padi serta ketahanannya terhadap patogen benih (Disertasi doktor, Universitas Hasanuddin). http://repository.unhas.ac.id:443/id/eprint/31748
Kokalis-Burelle, N., Vavrina, C. S., Rosskopf, E. N., & Shelby, R. A. (2002). Field evaluation of plant growth-promoting rhizobacteria amended transplant mixes and soil solarization for tomato and pepper production in Florida. Plant and Soil, 238(2), 257–266. https://doi.org/10.1023/A:1014464716261
Komansilan, O., Paulus, J. M., & Rogi, J. E. (2022). Pemberian Plant Growth Promoting Rhizobacteria (PGPR) untuk meningkatkan produksi padi gogo dan jagung dalam sistem tumpang sari. Jurnal MIPA, 12(1), 1–10.
Kurniawan, I., et al. (2021). Pengaruh model jarak tanam jajar legowo terhadap pertumbuhan dan hasil padi (Oryza sativa) varietas IPB 3S. Daun: Jurnal Ilmiah Pertanian dan Kehutanan, 8(2), 98–109. https://doi.org/10.33084/daun.v8i2.2865
Lugtenberg, B., & Kamilova, F. (2009). Plant-growth-promoting rhizobacteria. Annual Review of Microbiology, 63, 541–556. https://doi.org/10.1146/annurev.micro.62.081307.162918
Muslim, I. A., & Suwandi, I. (2023). Pengendalian hayati patogen tanaman dengan mikroorganisme antagonis (Edisi revisi). UPT Penerbit dan Percetakan Universitas Sriwijaya.
Pan, J. Y., Wang, C. B., Nong, J. L., Xie, Q. L., & Shen, T. M. (2023). Plant growth-promoting rhizobacteria are important contributors to rice yield in karst soils. 3 Biotech, 13(5), Article 158. https://doi.org/10.1007/s13205-023-03578-0
Purwanto, P., Oktaviani, E., & Leana, N. W. A. (2022). Seed bio-priming to enhance seed germination and seed vigor of rice using rhizobacteria from the northern coast of Pemalang, Central Java, Indonesia. Planta Tropika: Journal of Agro Science, 10(2), 152–159. https://doi.org/10.18196/pt.v10i2.14934
Richardson, A. E., Barea, J. M., McNeill, A. M., & Prigent-Combaret, C. (2009). Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant and Soil, 321(1–2), 305–339. https://doi.org/10.1007/s11104-009-9895-2
Romadhona, F. N., Mariati, N. D., Laksamana, B., & Dukat, D. (2025). Effect of Plant Growth Promoting Rhizobacteria (PGPR) on the growth and yield of rice plants (Oryza sativa L.). Jurnal Agrosci, 3(1), 54–67. https://doi.org/10.62885/agrosci.v3i1.896
Sandiase, I. K., Widiyanti, N. L. P. M., & Warpala, I. W. S. (2023). Variasi konsentrasi Plant Growth Promoting Rhizobacteria (PGPR) rendaman akar bambu menghambat pertumbuhan jamur Fusarium oxysporum secara in vitro. Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati, 120–130. https://doi.org/10.24002/biota.v8i2.6075
Taiz, L., & Zeiger, E. (2014). Plant physiology and development (6th ed.). Sinauer Associates Sunderland. MA, USA.
Vessey, J. K. (2003). Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil, 255(2), 571–586. https://doi.org/10.1023/A:1026037216893
Wang, Y., Chen, Z., Wu, F., & Sui, N. (2026). From rhizosphere to endosphere: Conserved and specialised mechanisms of plant growth-promoting bacteria in mediating salt tolerance. Plant, Cell & Environment. https://doi.org/10.1111/pce.70616
Wang, Y., Pei, Y., Wang, X., Dai, X., & Zhu, M. (2024). Antimicrobial metabolites produced by the plant growth-promoting rhizobacteria (PGPR): Bacillus and Pseudomonas. Advanced Agrochem. https://doi.org/10.1016/j.aac.2024.100088
Wati, T. A. P., Aswidinnoor, H., Surahman, M., & Nugroho, D. A. (2019). Demonstrasi plot padi IPB 3S dan IPB 9G sebagai upaya peningkatan pengetahuan petani Desa Mekarharja, Kecamatan Purwaharja, Kota Banjar. https://journal.ipb.ac.id/index.php/pim/article/view/28421
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